DOI: 10.25881/BPNMSC.2021.29.11.020


Churashov S.V., Zlobin I.A., Chernysh V.F., Malafeeva A.Yu.

S.M. Kirov Military medical academy, St. Petersburg


The main causes of limbal stem cells deficiency (LSCD) in eyes with vascular leucorrhoea are burns, trauma, chronic inflammation, scarring processes, irregularities in the moisture of the ocular surface, excessive surgical activity in the limbal zone, etc. The answer to the question about the phenotype of the epithelium covering the corneal leucorrhoea can be given by the method of impression cytology.

Aim: To assess the information content of the method of impression cytology in the diagnosis of LSCD in eyes with vascular corneal leucorrhoea.

Materials and methods: The study included 35 patients (35 eyes) aged 25 to 80 years with total vascular corneal leucorrhoea. The reasons for the formation of vascular leucorrhoea in the eyes were: endothelial-epithelial dystrophy in stage IV (n = 10); recurrent metaherpetic keratitis (n = 5); recurrence of thorns after traditional optical penetrating keratoplasty (n = 10); severe chemical burns (n = 10). An improved method of impression cytology was used to determine the phenotype of the surface epithelium of the leucorrhoea.

Keywords: cornea, limbal stem cells deficiency, impression cytology.


1. Neroev VV. Organization of ophthalmological care for the population of the Russian Federation. Bulletin ophthalmology. 2014; 130(6): 8–12. (In Russ)]

2. World Health Organization. Global data on visual impairments 2010. World Health Organization. Geneva: WHO, 2012: 17.

3. Chernysh VF, Boiko EV. Eye burns — the state of the problem and new approaches. SPb: VMA; 2008: 133 (In Russ).

4. Dua HS. Limbal stem cell deficiency: concept, aetiology, clinical presentation, diagnosis and management. Indian J of Ophthalmol. 2000; 48(2): 83–92.

5. Holland EJ. The evolution of epithelial transplantation for severe ocular surface disease and a proposed classification system. Cornea. 1996; 15: 549–556.

6. Tanaeva KhD. Limbal transplantation as a method of induction of local immune tolerance in high-risk keratoplasty. Bulletin of Transplantology and Artificial Organs. 2011; 8(2): 95–102. (In Russ).

7. Global consensus on definition, classification, diagnosis, and staging of limbal stem cell deficiency. Cornea. 2019; 38(3): 364–437. doi: 10.1097/ICO.0000000000001820.

8. Fedorov AA. Morphological foundations of scientific research in ophthalmology. Bulletin of ophthalmology. 2013; 5: 10–22. (In Russ).

9. Shapiro NA. Principles of cytological diagnosis of malignant tumors: color atlas. Reprocentr M; 2008: 350. (In Russ).

10. Egbert PR. A simple conjunctival biopsy. Am. J. Ophthalmol. 1977; 84(6): 798–801. doi: 10.1016/0002-9394(77)90499-8.

11. Nelson JD. Impression cytology. Cornea. 1988; 7(1): 71–81.

12. Singh R. Impression cytology of the ocular surface. Br. J. Ophtalmol. 2005; 89: 1655–59. doi: 10.1136 / bjo.2005.073916.

13. Gavrilyuk IO, Kulikov AN, Chernysh VF et al. Improvement of the method of preparation of preparations of the corneal epithelium for impression cytology for the purpose of vital assessment of its phenotype in the experiment. Modern technologies in ophthalmology. 2017; 4: 55–57. (In Russ).

14. Zuazo F. Conjunctival impression cytology in patients with normaland impaired OSDI scores. Arch.soc. esp. oftalmol. 2014; 89(10): 391–396. doi: 10.1016/ j.oftal.2014.04.013.

For citation

Churashov S.V., Zlobin I.A., Chernysh V.F., Malafeeva A.Yu. Diagnosis of limbal stem cells deficiency in eyes with total vascular bellams by impression cytology. Bulletin of Pirogov National Medical & Surgical Center. 2021;16(1):112-114. (In Russ.)