DOI: 10.25881/BPNMSC.2020.30.34.015

Authors

Krupina E.A., Fajzrahmanov R.R., Pavlovskij O.A., Larina E.A., Suhanova A.V., Karpov G.O.

Pirogov National Medical and Surgical Center, Moscow

Abstract

Biological therapies have attracted more and more attention in recent decades. Knowledge of the basic biological mechanisms involved in tissue response to injury should inform management of healing. Approaches to influence healing may need to integrate multiple cell types and large signaling networks that are necessary for the dynamic communication between cells. To date, in medicine, widespread therapies based on the use of autologous blood plasma (ABP). In this study, a biological analysis of platelet-rich plasma was performed. obtained using tubes for ABP-therapy of two types. In the first group, test tubes were used to obtain PRP, in the second to obtain ACP.

The study showed that when choosing tubes for PRP therapy, it is important to take into account the centrifugation mode and the plasma selection system, preferring closed systems that allow to obtain pure ABP, preserve sterility and reduce the risks of contamination of biomaterial with a minimum number of manipulations. In particular, the therapy of inflammatory nosologies requires the use of PRP, which may determine the possibility of correcting the local immune status. In cavity operations in ophthalmology, it is advisable to use ACP, which has a low response from the immune system. The main role of APC in this case tamponade tissue defect due to the properties of the material used. Identifyingwhich molecular mechanisms are more or less important during the course of healing andclarifying the molecular basis for differences in the healing response across patients willcontinue to be the priority to tailor PRP therapies for particular sports injuries.

Keywords: PRP therapy, ACP therapy, macular hole.

References

1. Arsyutov DG. Ispol'zovanie autologichnoi konditsionirovannoi plazmy, obogashchennoi trombotsitami, v khirurgii regmatogennoi otsloiki setchatki s tsentral'nym i perifericheskimi razryvami. Acta Biomedica Scientifica. 2019;4(4).

2. Akhmerov R, Zarudii R, Rychkova I. Plazmolifting. Lechenie vozrastnoi atrofii kozhi bogatoi trombotsitami autoplazmoi. Esteticheskaya meditsina. 2011;(2):181–187.

3. Achkasov EE, Bezuglov EN, Ul'yanov AA. Primenenie autoplazmy, obogashchennoi trombotsitami, v klinicheskoi praktike. Biomeditsina. 2013;(4):46–59.

4. Izmailova TA. Personalizirovannaya PRP-terapiya: algoritm podgotovki patsienta. In"ektsionnye metody v kosmetologii. 2016;(2):76–81.

5. Faizrakhmanov RR. Rezhimy naznacheniya anti-VEGF-preparatov pri terapii neovaskulrnoi vozrastnoi makulyarnoi degeneratsii. Vestnik oftal'mologii. 2018;(6):105–113. Doi: 10.17116/oftalma2018134061105.

6. Faizrakhmanov RR. Anti-VEGF terapiya neovaskulyarnoi vozrastnoi makulyarnoi degeneratsii: ot randomizirovannykh issledovanii k real'noi klinicheskoi praktike. Rossiiskii oftal'mologicheskii zhurnal. 2019;(12):97–105. Doi: 10.21516/2072-0076-2019-12-2-97-105.

7. Faizrakhmanov RR. Ozurdeks v terapii diabeticheskogo makulyarnogo oteka. Kogda naznachat'. Vestnik oftal'mologii. 2019;(4):121–125. Doi: 10.17116/oftalma2019135041121.

8. Faizrakhmanov RR. Pavlovskii O.A., Larina E.A. Khirurgicheskoe lechenie patsientov s neustranennym makulyarnym razryvom. Vestnik Natsional'nogo mediko-khirurgicheskogo Tsentra im. N.I. Pirogova. 2019;(14):98–104. Doi: 10.25881/BPNMSC.2018.64.37.021.

9. Shpak AA, Shkvorchenko DO, Krupina EA. Strukturnye izmeneniya makulyarnoi oblasti posle khirurgicheskogo lecheniya makulyarnykh razryvov s primeneniem bogatoi trombotsitami plazmy krovi. Oftal'mokhirurgiya. 2018;(4):52–57.

10. Anitua Е. Preservation of biological activity of plasma and platelet-derived eye-drops after their different time and temperature conditions of storage. Cornea. 2015;34(9):1144–1148.

11. Arshdeep К, Kumaran М. Platelet-rich plasma in dermatology: Boon or a bane. Ind J Dermatol Venereol Leprol. 2014;80:5–14.

12. Dragonas P, Katsaros T, Avila-Ortiz G, et al. Effects of leukocyte-platelet-rich fibrin (L-PRF) in different intraoral bone grafting procedures: a systematic review. Int J Oral Maxillofac Surgery. 2019;48(2)250–262.

13. Ehrenfest DM, Bielecki T, Mishra A, et al. In search of a consensus terminology in the field of platelet concentrates for surgical use: plateletrich plasma (PRP), platelet-rich fibrin (PRF), fibrin gel polymerization and leukocytes. Current pharmaceutical biotechnology. 2012;13(7):1131–1137.

14. Soldatova L, Campbell RG, Elkhatib AH, et al. Role of Leukocyte-Platelet-Rich Fibrin in Endoscopic Endonasal Skull Base Surgery Defect Reconstruction. J Neurol Surgery Skull Base. 2017;78(1):59–62. Doi: 10.1055/s-0036-1584894.

15. Yang B, Houlberg K, Millward A, et al. Polymorphisms of chemokine and chemokine receptor genes in Type 1 diabetes mellitus and its complications. Cytokine. 2004;7(26):114–121.

For citation

Krupina E.A., Fajzrahmanov R.R., Pavlovskij O.A., Larina E.A., Suhanova A.V., Karpov G.O. Molecular and Biological аspects of Platelet-Rich Plasma Therapies. Bulletin of Pirogov National Medical & Surgical Center. 2020;15(3-2):80-85. (In Russ.) https://doi.org/10.25881/BPNMSC.2020.30.34.015